Merck
CN

G1160

Sigma-Aldrich

单克隆抗- 谷胱甘肽-S-转移酶(GST) 小鼠抗

clone GST-2, ascites fluid

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别名:
Mouse Anti-GST Tag
MDL编号:
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

ascites fluid

antibody product type

primary antibodies

克隆

GST-2, monoclonal

包含

15 mM sodium azide

technique(s)

dot blot: suitable
indirect ELISA: suitable
western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST

同位素/亚型

IgG2b

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
SAB4200237SAB4200692A7340
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

peroxidase conjugate

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

clone

GST-2, monoclonal

clone

2H3-D10, monoclonal

clone

GST-2, monoclonal

clone

polyclonal

technique(s)

dot blot: suitable, indirect ELISA: suitable, western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST

technique(s)

western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane

technique(s)

ELISA: suitable, dot blot: suitable, immunoblotting: 0.5 μg/mL, immunofluorescence: suitable, immunoprecipitation (IP): suitable

technique(s)

direct ELISA: 1:10,000, western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST

isotype

IgG2b

isotype

-

isotype

IgG2b

isotype

-

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一般描述

单克隆抗谷胱甘肽-S-转移酶(GST)(小鼠IgG2b同种型)来自GST-2杂交瘤,该杂交瘤通过用纯化的重组 GST 融合蛋白免疫 BALB/c 小鼠,取其脾细胞与小鼠骨髓瘤细胞融合产生。GST 属于三个蛋白家族,分别是胞质GST、线粒体GST和微粒体GST。目前,已鉴定出下列八个不同类别的哺乳动物可溶性胞质谷胱甘肽S-转移酶:α、κ、μ、ω、σ、θ、π和ζ。
通过各种表达载体构建体,重组靶蛋白通常表达为带谷胱甘肽-S-转移酶(GST)标签的融合产物。因此,直接对应重组构建体 GST 标签的抗体可用于靶蛋白的纯化和研究。单克隆抗谷胱甘肽-S-转移酶 (GST) 抗体与从日本血吸虫( Schistosoma japonicum)中提取的 GST 反应。此外,该产品还可以鉴定纯化 GST 和 GST 融合蛋白的天然形式以及变性还原形式。该抗体不能检测来自大鼠、兔子、猪和牛肝或人胎盘的 GST。

免疫原

重组谷胱甘肽-S-转移酶 (GST) 融合蛋白。

应用

谷胱甘肽-S-转移酶(GST)单克隆抗体可用于蛋白印迹和ELISA。此产品也可用于斑点印迹。

生化/生理作用

谷胱甘肽 S-转移酶(GST)是一类酶家族,通过催化许多疏水性化合物和亲电化合物与还原型谷胱甘肽结合,在解毒中发挥重要作用。

免责声明

除非我们的目录或产品随附的其他公司文档中另有说明,我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人或动物的任何类型的消费或应用。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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