G1270
L-谷氨酰胺合成酶 来源于大肠杆菌
lyophilized powder, 400-2,000 units/mg protein
别名:
L-Glutamate:ammonia ligase (ADP-forming)
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.26
MDL number:
Specific activity:
400-2,000 units/mg protein
form
lyophilized powder
Quality Segment
specific activity
400-2,000 units/mg protein
purified by
affinity chromatography
contains
dithioerythritol as preservative
composition
Protein, ~5% Lowry
solubility
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
UniProt accession no.
foreign activity
ATPase <0.2%
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... glnA(948370)
General description
L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.
Application
L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.
Biochem/physiol Actions
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
谷氨酸降解酶
Physical form
Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts
Other Notes
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)