G3144
L-谷氨酰胺合成酶 来源于大肠杆菌 W，ATCC 号9637
Protein ≥30 % by biuret, lyophilized powder
别名:
L-谷氨酸:氨连接酶（形成 ADP）
表单
lyophilized powder
比活
100-400 units/mg protein
组成
Protein, ≥30% biuret
溶解性
H2O: soluble 0.95-1.05 mg/mL, clear to slightly hazy
UniProt登记号
储存温度
−20°C
基因信息
Escherichia coli K12 ... glnA(948370)
应用
L-谷氨酰胺合成酶可用于从大肠杆菌中纯化蛋白酶。
生化/生理作用
L-谷氨酰胺合成酶催化L-谷氨酸和氨缩合为L-谷氨酰胺。它是谷氨酸的降解酶。
谷氨酸降解酶
外形
含缓冲盐和稳定剂的冻干粉
制备说明
在含葡萄糖和 NH4Cl 的培养基中生长
其他说明
一个单位将在37℃，pH值7.1下于15分钟内将1.0μ摩尔的L-谷氨酸转化为L-谷氨酰胺。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
H S Kingdon et al.
Journal of bacteriology, 94(4), 949-957 (1967-10-01)
The kinetic properties of Escherichia coli glutamine synthetase are markedly influenced by the manner in which the organism is grown. Enzyme obtained from stationary-phase cells grown on glycerol and glutamate is strongely inhibited by each of the eight feedback effectors
Beatriz Sánchez-Pardo et al.
Journal of plant physiology, 170(3), 265-271 (2012-12-19)
The aims of this work were to investigate the microlocalisation of cadmium (Cd) in Lupinus albus L. cv. Multolupa nodules, and to determine its effects on carbon and nitrogen metabolism. Nodulated white lupin plants were grown in a growth chamber
Peritumoral hyperplasia of the liver: a response to portal vein invasion by hypervascular neoplasms.
Thomas Arnason et al.
Histopathology, 62(3), 458-464 (2012-12-18)
Several cases of focal nodular hyperplasia (FNH) or similar hyperplastic lesions have been reported adjacent to hepatic neoplasms, including hepatocellular carcinoma, epithelioid haemangioendothelioma and hepatoblastoma. We refer to this hyperplastic response as peritumoral hyperplasia (PTH). Here, we report eight cases
Purification of a protease from Escherichia coli with specificity for oxidized glutamine synthetase.
J E Roseman et al.
The Journal of biological chemistry, 262(5), 2101-2110 (1987-02-15)
A soluble Escherichia coli protease has been identified and purified to homogeneity. The protease cleaves glutamine synthetase which has been modified by mixed function oxidation; native glutamine synthetase is not a substrate. Using [14C]glutamine synthetase as a substrate (prepared by
Kamal Krishna Singh et al.
Plant cell reports, 32(2), 183-193 (2012-10-17)
KEY MESSAGE : The regulation of GS isoforms by WD was organ specific. Two GS isoforms i.e. OsGS1;1 and OsGS2 were differentially regulated in IR-64 (drought-sensitive) and Khitish (drought-tolerant) cultivars of rice. Water deficit (WD) has adverse effect on rice
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