Anti-GRP75 (KA-20) antibody produced in rabbit

GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing. GRP75/mortalin has been localized mainly to the endoplasmic reticulum (ER), but also to various cellular compartments including mitochondria and cytoplasmic vesicles.

synthetic peptide corresponding to amino acids 617-636 located near the C-terminus of human GRP75/mortalin-2. This sequence is identical in rat GRP75 and highly conserved in chicken (1 amino acid substitution) and mouse (2 amino acids substitutions) GRP75.

Anti-GRP75 antibody produced in rabbit is suitable for microarray and immunoblotting at a working antibody concentration of 0.2-0.4μg/mL using whole cell extracts of the human epitheloid carcinoma HeLa cells, Madin-Darby canine kidney (MDCK) cells, and mouse fibroblasts NIH3T3 cells.

GRP75 (glucose-regulated protein 75) belongs to a subfamily of the heat shock proteins Hsp70. It is involved in stress responses to intracellular trafficking, antigen processing, and control of cell proliferation, differentiation, and tumorigenesis. It is induced by low levels of ionizing radiation, glucose deprivation, calcium ionophore, and ozone and its levels correlate with muscle activity, mitochondrial activity, and biogenesis.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.