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Merck
CN

G4385

Glutathione S-Transferase from bovine liver

lyophilized powder, 10-20 units/mg protein

别名:

Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase

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UNSPSC Code:
12352200
MDL number:
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form

lyophilized powder

specific activity

10-20 units/mg protein

composition

Protein, ~60%

UniProt accession no.

storage temp.

−20°C

Gene Information

Biochem/physiol Actions

Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.
Protein family catalyzing conjugation of reduced glutathione with various hydrophobic chemicals.

Physical form

Lyophilized powder containing Tris and phosphate buffer salts, reduced glutathione and EDTA

Analysis Note

Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.

Other Notes

One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

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