form
lyophilized powder
specific activity
10-20 units/mg protein
composition
Protein, ~60%
storage temp.
−20°C
Gene Information
cow ... GSTA2(281805), GSTA4(533917), GSTM1(327709)
Biochem/physiol Actions
Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.
Protein family catalyzing conjugation of reduced glutathione with various hydrophobic chemicals.
Physical form
Lyophilized powder containing Tris and phosphate buffer salts, reduced glutathione and EDTA
Analysis Note
Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.
Other Notes
One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.
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