G5758
葡萄糖-6-磷酸酶 来源于兔肝脏
≥0.05 units/mg protein, Protein ~30 % (balance mostly sucrose)
别名:
D-葡萄糖6-磷酸磷酸水解酶
form
powder with sucrose
specific activity
≥0.05 units/mg protein
composition
Protein, ~30% (balance mostly sucrose)
storage temp.
−20°C
General description
葡萄糖-6-磷酸酶是一种多组分蛋白质复合物，由葡萄糖-6-磷酸转运蛋白（G6PT）和催化单元（G6PC）组成。肝和肾细胞具有葡萄糖-6-磷酸酶的主要表达。
Application
来自兔肝的葡萄糖-6-磷酸酶已被用于粗制桔梗叶提取物以及阿托维德多杀菌素的甲醇性叶和树皮提取物中的葡萄糖6-磷酸酶抑制剂检测。
葡萄糖-6-磷酸酶已被用于研究黄皮属茴香（野钩叶）提取物对某些糖尿病相关代谢酶的影响。
Biochem/physiol Actions
葡萄糖-6-磷酸酶可催化葡萄糖-6-磷酸转化为葡萄糖，作为糖异生的最终步骤。
葡萄糖-6-磷酸酶对于在饥饿期间调节葡萄糖的稳态至关重要。葡萄糖-6-磷酸酶的缺乏与糖原贮积病I型（GSDI）有关。
Analysis Note
粗微粒体制备
Other Notes
据报道，葡萄糖-6-磷酸酶、无机焦磷酸酶和焦磷酸葡萄糖磷酸转移酶与可催化三种不同反应的单一蛋白相关：
a) 葡萄糖6-磷酸 + H2O → 葡萄糖 + Pi
b) PPi + H2O → 2 Pi
c) 葡萄糖 + PPi或核苷酸-DP 或 -TP → 葡萄糖6-磷酸 + Pi或核苷酸-MP。
a) 葡萄糖6-磷酸 + H2O → 葡萄糖 + Pi
b) PPi + H2O → 2 Pi
c) 葡萄糖 + PPi或核苷酸-DP 或 -TP → 葡萄糖6-磷酸 + Pi或核苷酸-MP。
在pH 6.5、37 °C条件下，一单位在每分钟可从葡萄糖6-磷酸释放1.0 μmole的无机磷酸。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Glucose-6-phosphatase catalytic subunit gene family
Hutton JC and O'Brien RM
The Journal of Biological Chemistry, 284(43), 29241-29245 (2009)
Emile van Schaftingen et al.
The Biochemical journal, 362(Pt 3), 513-532 (2002-03-07)
Glucose-6-phosphatase (G6Pase), an enzyme found mainly in the liver and the kidneys, plays the important role of providing glucose during starvation. Unlike most phosphatases acting on water-soluble compounds, it is a membrane-bound enzyme, being associated with the endoplasmic reticulum. In
The effects of Clausena anisata (Wild) Hook leaf extracts on selected diabetic related metabolizing enzymes
Mogale MA, et al.
Journal of Medicinal Plants Research, 6(25), 4200-4207 (2012)
Effects of Quillaja saponins on growth, feed efficiency, digestive enzyme activities and metabolism of common carp (Cyprinus carpio L)
Serrano Jr, AE
Aquaculture Nutrition, 19(4), 468-474 (2013)
EVIDENCE FOR THE COMMON IDENTITY OF GLUCOSE 6-PHOSPHATASE, INORGANIC PYROPHOSPHATASE, AND PYROPHOSPHATE-GLUCOSE PHOSPHOTRANSFERASE.
R C NORDLIE et al.
The Journal of biological chemistry, 239, 1680-1685 (1964-06-01)
实验方案
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
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