G5758
葡萄糖-6-磷酸酶 来源于兔肝脏
≥0.05 units/mg protein, Protein ~30 % (balance mostly sucrose)
别名:
D-葡萄糖6-磷酸磷酸水解酶
form
powder with sucrose
specific activity
≥0.05 units/mg protein
composition
Protein, ~30% (balance mostly sucrose)
storage temp.
−20°C
General description
葡萄糖-6-磷酸酶是一种多组分蛋白质复合物，由葡萄糖-6-磷酸转运蛋白（G6PT）和催化单元（G6PC）组成。肝和肾细胞具有葡萄糖-6-磷酸酶的主要表达。
Application
来自兔肝的葡萄糖-6-磷酸酶已被用于粗制桔梗叶提取物以及阿托维德多杀菌素的甲醇性叶和树皮提取物中的葡萄糖6-磷酸酶抑制剂检测。
葡萄糖-6-磷酸酶已被用于研究黄皮属茴香（野钩叶）提取物对某些糖尿病相关代谢酶的影响。
Biochem/physiol Actions
葡萄糖-6-磷酸酶可催化葡萄糖-6-磷酸转化为葡萄糖，作为糖异生的最终步骤。
葡萄糖-6-磷酸酶对于在饥饿期间调节葡萄糖的稳态至关重要。葡萄糖-6-磷酸酶的缺乏与糖原贮积病I型（GSDI）有关。
Analysis Note
粗微粒体制备
Other Notes
据报道，葡萄糖-6-磷酸酶、无机焦磷酸酶和焦磷酸葡萄糖磷酸转移酶与可催化三种不同反应的单一蛋白相关：
a) 葡萄糖6-磷酸 + H2O → 葡萄糖 + Pi
b) PPi + H2O → 2 Pi
c) 葡萄糖 + PPi或核苷酸-DP 或 -TP → 葡萄糖6-磷酸 + Pi或核苷酸-MP。
a) 葡萄糖6-磷酸 + H2O → 葡萄糖 + Pi
b) PPi + H2O → 2 Pi
c) 葡萄糖 + PPi或核苷酸-DP 或 -TP → 葡萄糖6-磷酸 + Pi或核苷酸-MP。
在pH 6.5、37 °C条件下，一单位在每分钟可从葡萄糖6-磷酸释放1.0 μmole的无机磷酸。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Glucotoxicity Induces Glucose-6-Phosphatase Catalytic Unit Expression by Acting on the Interaction of Hypoxia Inducible Factor-1alpha With cAMP-Responsive Element-Binding Protein-Binding Protein
Gautier-Stein A, et al.
Diabetes, DB_110986-DB_110986 (2012)
Glucose-6-phosphatase deficiency
Froissart R, et al.
Orphanet Journal of Rare Diseases, 6(1), 27-27 (2011)
Yan Wang et al.
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 14(11), 856-858 (2012-11-14)
To investigate the mutation of glucose-6-phosphatase gene (G6PC gene) in a patient with glycogen storage disease Ⅰa. PCR was used to amplify all five exons of G6PC gene. The PCR products were directly sequenced to detect the mutations. A heterozygous
Bidur Bhandary et al.
Biological & pharmaceutical bulletin, 35(11), 1907-1913 (2012-11-06)
Hyperlipidemia is a major contributor for atherosclerosis and hypolipidemic drugs such as statin are highly prescribed to treat elevated lipid level in plasma. Rubus coreanus, which is widely cultivated in south eastern Asia, have been reported to show significant cholesterol
Glucose-6-phosphatase catalytic subunit gene family
Hutton JC and O'Brien RM
The Journal of Biological Chemistry, 284(43), 29241-29245 (2009)
实验方案
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
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