特点
autoclavable Autoclavable, 20 min at 120°C in pH 7
包装
pack of 1 L
制造商/商品名称
Cytiva 17-0110-01
基质
6% agarose
粒径
45-165 μm
cleaning in place
4-9
工作范围
4-9
SMILES字符串
OC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@@H](O3)O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H]6CO[C@H]5[C@H](O)[C@H](O)O6)[C@@H]4O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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一般描述
Sepharose™ 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.
特点和优势
- 6% Agarose gel filtration media.
- Proven base matrix for coupling affinity ligands
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
珠状琼脂糖用于分离高分子量的分子。交联珠状琼脂糖更能耐受变性条件，从而为样本缓冲液和洗脱液的选择提供更多的灵活性。近似的 % 琼脂糖浓度由产品名 (Ultrogel A%) 表示。
法律信息
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
此项目有
P Upcroft et al.
Journal of chromatography, 618(1-2), 79-93 (1993-08-25)
Agarose as a medium for separation of DNA was first introduced in 1962 and since the early 1970s agarose submarine gel electrophoresis has been synonymous with separations of DNA molecules larger than 1 kilobase pair (kb). The large pore size
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
N C Stellwagen et al.
Electrophoresis, 14(4), 355-368 (1993-04-01)
The orientation of the agarose gel matrix in pulsed electric fields has been studied by transient electric birefringence. Two types of agarose with different degrees of charge were studied, in addition to agarose solutions and gels containing beta-carrageenan, a stereoisomer
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized
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