InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
SMILES string
OC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@@H](O3)O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H]6CO[C@H]5[C@H](O)[C@H](O)O6)[C@@H]4O)[C@H](O)[C@@H](O)[C@H]1O
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
feature
autoclavable Autoclavable, 20 min at 120°C in pH 7
packaging
pack of 1 L
manufacturer/tradename
Cytiva 17-0110-01
matrix
6% agarose
particle size
45-165 μm
cleaning in place
4-9
working range
4-9
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General description
Sepharose™ 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.
Features and Benefits
- 6% Agarose gel filtration media.
- Proven base matrix for coupling affinity ligands
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
珠状琼脂糖用于分离高分子量的分子。交联珠状琼脂糖更能耐受变性条件，从而为样本缓冲液和洗脱液的选择提供更多的灵活性。近似的 % 琼脂糖浓度由产品名 (Ultrogel A%) 表示。
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
此项目有
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized
Purification of recombinant protein derived from the baculovirus expression system using glutathione affinity agarose.
E J Sorscher et al.
Methods in molecular biology (Clifton, N.J.), 39, 337-348 (1995-01-01)
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
N C Stellwagen et al.
Electrophoresis, 14(4), 355-368 (1993-04-01)
The orientation of the agarose gel matrix in pulsed electric fields has been studied by transient electric birefringence. Two types of agarose with different degrees of charge were studied, in addition to agarose solutions and gels containing beta-carrageenan, a stereoisomer
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
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