包装
pack of 15 g
制造商/商品名称
Cytiva 17-0640-01
基质
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
2-8
工作范围
2-8
储存温度
2-8°C
一般描述
Activated Thiol Sepharose™ 4B medium is a medium used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules.
Activated Thiol Sepharose™ 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose™ 4B. Activated Thiol Sepharose™ 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules.
Activated Thiol Sepharose™ 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose™ 4B. Activated Thiol Sepharose™ 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules.
特点和优势
- Reversible coupling of proteins and large biomolecules with thiol groups to Sepharose™ 4B via a glutathione spacer arm
- The ligand is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBractivated Sepharose™ 4B.
- Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids
- Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
- Separate thiol-containing proteins from non-thiol-containing proteins
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
储存分类代码
13 - Non Combustible Solids
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