packaging
pack of 15 g
manufacturer/tradename
Cytiva 17-0640-01
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-8
working range
2-8
storage temp.
2-8°C
General description
Activated Thiol Sepharose™ 4B medium is a medium used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules.
Activated Thiol Sepharose™ 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose™ 4B. Activated Thiol Sepharose™ 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules.
Activated Thiol Sepharose™ 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose™ 4B. Activated Thiol Sepharose™ 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules.
Features and Benefits
- Reversible coupling of proteins and large biomolecules with thiol groups to Sepharose™ 4B via a glutathione spacer arm
- The ligand is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBractivated Sepharose™ 4B.
- Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids
- Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
- Separate thiol-containing proteins from non-thiol-containing proteins
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
存储类别
13 - Non Combustible Solids
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实验方案
This page shows how to prepare samples for purification with affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
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