Lysine-Sepharose^™ 4B

Lysine Sepharose^™ 4B media with immobilized amino acid lysine is mainly used for isolation of plasminogen and plasminogen activator. The ε-amino and α-carboxyl groups on lysine is free after immobilization and interact with a range of biomolecules.

Lysine Sepharose^™ 4B is L-lysine immobilized to Sepharose^™ 4B by the cyanogen bromide method. L-lysine is coupled via its α-amino group, leaving both the ε-amino and α-carboxyl groups free to interact with sample substances during chromatography. It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine. Lysine Sepharose^™ 4B is a group specific adsorbent and has been used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA. The precise mechanism by which separation occurs is not fully understood but both electrostatic and stereospecific effects may contribute to the separation, depending upon the application.

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

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Sepharose is a trademark of Cytiva