packaging
pack of 1 ea, pkg of 1 ea
manufacturer/tradename
Cytiva 17-1150-01
availability
not available in Japan
storage temp.
room temp
General description
PlusOne Silver Staining Kit, Protein and PlusOne DNA Silver Staining Kit are two silver staining kits that bring speed, convenience, and very high sensitivity to the staining of proteins and nucleic acids in polyacrylamide electrophoresis. gels.
Silver Staining Kit, Protein includes detailed protocols and components in quantities sufficient to stain 10 DALT precast gels, 10 precast 12.5 x 26 cm gels, 20 mini slab gels (8–10 x 8 cm), or 30 to 60 PhastGels. It can also be used with the Processor Plus.
The kit contains the following components: sodium acetate, 5% sodium thiosulfate, EDTA-Na2, sodium carbonate, 2.5% silver nitrate, 37% formaldehyde, and 25% glutaraldehyde.
Silver Staining Kit, Protein includes detailed protocols and components in quantities sufficient to stain 10 DALT precast gels, 10 precast 12.5 x 26 cm gels, 20 mini slab gels (8–10 x 8 cm), or 30 to 60 PhastGels. It can also be used with the Processor Plus.
The kit contains the following components: sodium acetate, 5% sodium thiosulfate, EDTA-Na2, sodium carbonate, 2.5% silver nitrate, 37% formaldehyde, and 25% glutaraldehyde.
Features and Benefits
- Based on the methodology of Heukeshoven and Dernick, the kit gives essentially colorless backgrounds in most gel electrophoresis systems.
- Silver Staining Kit, Protein provides convenient, reproducible and sensitive staining of proteins in polyacrylamide gels .including PhastGels in less than two hours
- The visualization technique detects most proteins in the nanogram range, which is 100-fold more sensitive than Coomassie Blue staining.
- Typical sensitivity is 0.2 to 0.6 ng protein/band.
- Use with 2-D electrophoresis and mass spectrometry by omitting glutaraldehyde from staining procedure.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiLoad is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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