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Merck
CN

GE17-1167-01

HiPrep™ 16/60 Sephacryl® S-300 HR

Cytiva 17-1167-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

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NACRES:
SB.52
UNSPSC Code:
41115700

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产品名称

HiPrep™ 16/60 Sephacryl® S-300 HR, Cytiva 17-1167-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

material

polypropylene

mol wt

10000-1500000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-1167-01

parameter

<1 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

Preparation Note

Store at 4 to 30 °C (20% Ethanol)
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.

General description

HiPrep™ 16/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

法规信息

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Lot/Batch Number

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实验方案

Sephacryl® Separation

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

使用Sephacryl®进行分离

本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。

相关内容

Separation Options with Sephacryl®

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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