material
borosilicate glass column, polypropylene filter
mol wt
5000-5,000,000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-5172-01
parameter
0.3-0.5 mL/min flow rate, 1.5 Mpa (217 psi), 75-500 mL sample volume
bed volume
24 mL
column L × I.D.
30 cm × 10 mm
matrix
agarose
matrix active group
agarose phase
avg. part. size
13 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superose® 6 10/300 GL are prepacked Tricorn columns for high-performance gel filtration with a broad protein fractionation range of 5 000 to 5 000 000.
Application
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
for high-resolution separations across exceptionally broad molecular-weight ranges
Features and Benefits
- Achieve high-resolution separations across exceptionally broad molecular-weight ranges.
- Easy, efficient scale-up from prepacked Superose® 10/300 GL columns to Superose® prep grade lab packs.
- Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose® for some lipids, peptides, and small aromatic compounds.
- Prepacked Tricorn columns are durable, high-performance galss columns for reliable and reproducible separations.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superose is a registered trademark of Cytiva
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