material
borosilicate glass column, polypropylene filter
mol wt
10000-600000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-5175-01
parameter
0.25-0.75 mL/min flow rate, 1.5 Mpa (217 psi), 25-500 mL sample volume
technique(s)
HPLC: suitable
bed volume
24 mL
column L × I.D.
30 cm × 10 mm
matrix
agarose-dextran composite
avg. part. size
13 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superdex® 200 10/300 GL is a prepacked gel filtration column for high-resolution, semipreperative and analytical separations of biomolecules. Superdex® 200 has a separation range for molecules with molecular weights between 10 000 and 600 000.
Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family, that provideexcellent reproducibility and durability in purification using a chromatography system.
Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family, that provideexcellent reproducibility and durability in purification using a chromatography system.
Application
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
for high-resolution, semipreperative and analytical separations of biomolecules
Features and Benefits
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Highly suitable for the polishing step in a purification procedure.
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns.
Preparation Note
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superdex is a registered trademark of Cytiva
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