nProtein A Sepharose^™ 4 Fast Flow

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

nProtein A Sepharose^™ 4 Fast Flow is native protein A coupled to Sepharose^™ 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose^™ 4 Fast Flow is native protein A coupled to the well established Sepharose^™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose^™ 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose^™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose^™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose^™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose^™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Sepharose is a trademark of Cytiva