Pezz 18 Expresion Vector

pEZZ 18 Protein A Gene Fusion Vector.

The phagemid pEZZ 18 contains the Protein A signal sequence and two synthetic “Z” domains based on the “B” IgG binding domain of Protein A. Proteins are expressed as fusions with the “ZZ” peptide and secreted into the aqueous culture medium under the direction of the Protein A signal sequence. They are easily purified using IgG Sepharose^™ 6 Fast FLow to which the “ZZ” domain binds tightly. Because of its folding properties, the 14 kDa “ZZ” peptide has little effect on folding of the fusion partner into a native conformation.

Expression is controlled by the lacUV5 and Protein A promoters and is not inducible. Elements of the Protein A gene provide the ATG and ribosome-binding sites. Stop codons must be provided by the insert.

M13 Universal Sequencing Primer can be used for both double-stranded and single-stranded sequencing. A protocol for production of single-stranded DNA is provided with the vector.

Inserts containing a stop codon will yield white colonies when grown on media containing X-gal.

Host(s). E. coli strains carrying a lac deletion but capable of a-complementation of lacZ′.

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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