packaging
pkg of 1 g
manufacturer/tradename
Cytiva 27-5543-02
shipped in
dry ice
storage temp.
−20°C
General description
Oligo(dT)-Cellulose is particularly useful to molecular biologists, since it simplifies the purification of polyadenylated mRNA. Each gram of dry powder binds between 80 and 100 A260 units of poly(A) in 10 mM Tris and 500 mM KCl.
Application
Affinity chromatography is the only purification technique that allows separation of biological molecules on the basis of either individual structure or biological function.
Features and Benefits
- High binding capacity and chemical stability
- Supplied as dried powder
- Each gram occupies a packed volume of ~ 2.5 to 3.5 mL when suspended in water and binds 80 to 100 A260 units of poly(A) in 10 mM Tris (pH 7.5) and 500 mM KCl.
- It contains approximately 40 mg of oligo(dT) per gram of cellulose, and the chains of dT range from 2 to 25 nucleotides in length.
- Oligo(dT)-Cellulose Type 7 contains few fines and therefore is compatible with column chromatography.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
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