产品名称
HiTrap® MabSelect™, Cytiva 28-4082-56, pack of 5 × 5 mL
ligand
recombinant protein A (E. coli)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-4082-56
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12
working range
3-10
capacity
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Analysis Note
Application
Features and Benefits
- HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
General description
Other Notes
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
实验方案
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to prepare samples for purification with affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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