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Merck
CN

GE28-4083-47

Ab Spintrap™

Cytiva 28-4083-47

别名:

affinity column, antibody spin column, chromatography column, mAb spin column, polyclonal spin column, prepacked column, protein G Sepharose HP, spin column

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NACRES:
NA.25
UNSPSC Code:
41106500

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ligand

recombinant protein G lacking albumin-binding region

packaging

pkg of 50 × 100 μL

manufacturer/tradename

Cytiva 28-4083-47

bed volume

100 μL

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.), >1 mg binding capacity(IgG/column)

storage temp.

2-8°C

相关类别

General description

Ab SpinTrap™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.

Features and Benefits

  • Rapid and convenient small-scale purification of IgG.
  • Protein G Sepharose™ High Performance in prepacked spin columns.
  • No filtration or centrifugation of different crude samples necessary for antibody purification.
  • Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

法规信息

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

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