feature
wetted part: no
packaging
pack of 16 columns
manufacturer/tradename
Cytiva 28-9031-30
storage condition
(20% Ehtanol), (20% Ethanol)
bed volume
800 μL
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-10.5
working range
4-9
capacity
>300 nmol/mL binding capacity(biotin/ml medium 6 mg biotinylated BSA/ml medium), binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)
storage temp.
2-8°C
General description
Streptavidin HP SpinTrap™ are prepacked, single-use spin columns and 96-well filter plates for the preparation of protein samples and enrichment of proteins of interest from clarified cell lysates and biological fluids.
Application
Streptavidin HP SpinTrap™ can be used for Protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the streptavidin and the Protein of interest is enriched through the affinity interaction with the antibody. The columns can also be used for the direct enrichment of Proteins that are biotinylated. By binding a specific biotinylated Protein to the column, Protein-Protein interactions can also be investigated.
Features and Benefits
- For simple, small-scale Protein enrichment through immobilized biotinylated biomolecules.
- Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin.
- Each column is packed with Streptavidin Sepharose™ High Performance for reproducibility and high performance.
- Easy scale-up with HiTrap® Streptavidin HP prepacked columns.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
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