跳转至内容
Merck
CN

GE28-9031-34

Protein G High Performance Spintrap™

Cytiva 28-9031-34, pack of 16 columns

别名:

Protein G Column

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.25
UNSPSC Code:
41106500

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

ligand

recombinant protein G lacking albumin-binding region

feature

wetted part: no

packaging

pack of 16 columns

manufacturer/tradename

Cytiva 28-9031-34

storage condition

(20% Ehtanol), (20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.), >1 mg binding capacity(human IgG/column)

storage temp.

2-8°C

相关类别

General description

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein G HP SpinTrap™ is a prepacked, single-use spin column containing protein G Sepharose™ High Performance.

Application

Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持