ligand
StrepTactin
packaging
pkg of 1 × 5 mL
manufacturer/tradename
Cytiva 28-9075-47
storage condition
(20% Ethanol)
parameter
<20 mL/min flow rate, 42 psi (H2O at room temperature.)
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
working range
7
capacity
~6 mg binding capacity (Strep II-tagged protein/ml medium)(H2O at room temperature.)
suitability
suitable for chromatography
storage temp.
2-8°C
General description
StrepTrap HP is a ready to use HiTrap® column prepacked with StrepTactin Sepharose™ High Performance for purifying Strep-tag II recombinant proteins.
Application
Tagged purification using the Strep-tag.
Features and Benefits
- Highly pure Strep-tag II recombinant proteins eluted in concentrated form and small volumes.
- Physiological conditions and mild elution preserves the activity of the target protein.
- Convenient, time-saving operation and reproducible results.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
StrepTrap is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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商品
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
实验方案
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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