material
borosilicate glass column, polypropylene filter
mol wt
3000-70000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9205-04
parameter
0.15-0.6 mL/min flow rate, 1.8 Mpa (261 psi), 4-50 mL sample volume
bed volume
3 mL
column L × I.D.
15 cm × 5 mm
matrix
dextran composite
avg. part. size
13 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superdex® 75 5/150 GL is a pre-packed, short glass gel filtration column for rapid protein homogeneity analysis or purity checks of biomolecules in the molecular weight range 3 000 to 70 000.
Application
For screening and purity check of proteins in the molecular weight range 10 000 to 600 000, like antibodies.
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
for high-resolution, semipreperative and analytical separations of biomolecules
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superdex is a registered trademark of Cytiva
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