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Merck
CN

GE28-9355-98

Dextrin Sepharose™ High Performance

Cytiva 28-9355-98, pack of 100 mL

别名:

FPLC column packing, MBP affinity matrix, affinity resin, maltose binding protein affinity polymer, stationary phase

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UNSPSC代码:
41106500
NACRES:
NA.56

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ligand

dextrin

包装

pack of 100 mL

制造商/商品名称

Cytiva 28-9355-98

储存条件

(20% Ehtanol)

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning in place

2-13

工作范围

>7

容量

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)

储存温度

2-8°C

相关类别

一般描述

Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
Dextrin Sepharose™ High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

应用

Dextrin Sepharose™ High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose™ High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

特点和优势

  • Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
  • The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
  • Physiological conditions and mild elution preserve target Protein activity
  • Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

Lot/Batch Number

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商品

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

实验方案

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

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