ligand
dextrin
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 28-9355-98
storage condition
(20% Ehtanol)
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning in place
2-13
working range
>7
storage temp.
2-8°C
capacity
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- βGal/ml medium)
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General description
Dextrin Sepharose™ High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).
Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-βGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
Application
Dextrin Sepharose™ High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose™ High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.
Features and Benefits
- Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
- The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
- Physiological conditions and mild elution preserve target Protein activity
- Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids
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商品
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
实验方案
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
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