material
borosilicate glass column, titanium frit
mol wt
1000-300000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 29-0362-25
availability
not available in USA, Canada and Puerto Rico
parameter
0.01-0.1 mL/min flow rate, 2.4 Mpa max. pressure (350 psi), 4-50 mL sample volume
column L × I.D.
30 cm × 3.2 mm
matrix active group
agarose phase
avg. part. size
11 μm
working range
3-12
separation technique
size exclusion (SEC)
General description
The Precision Column PC 3.2/30 (2.4 ml), prepacked with Superose12, is designed for sensitive and highly resolving gel filtration separations of biomolecules in the micropreparative scale. Superose 12 has a broad fractionation range for molecular weights between 1000 and 300 000.
Application
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place). Columns are not suitable for use with ÄKTAprime plus system.
Features and Benefits
- Achieve high-resolution separations across exceptionally broad molecular-weight ranges with Superose gel filtration medium.
- Precision columns (PC) for micropurification and analysis: sample volumes of 4 - 50 μL.
- Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose for some lipids, peptides, and small aromatic compounds.
- New hardware design for convenient direct connection to high performance chromatography systems such as ÄKTA design.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superose is a registered trademark of Cytiva
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