material
borosilicate glass column, titanium frit
mol wt
10000-600000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 29-0362-32
availability
not available in USA, Canada and Puerto Rico
parameter
<50 mL sample volume, 0.01-0.1 mL/min flow rate, 1.5 Mpa max. pressure (217 psi)
column L × I.D.
30 cm × 3.2 mm
avg. part. size
13 μm
working range
3-12
separation technique
size exclusion (SEC)
General description
Superdex 200 3.2/300 is a prepacked Precision Column (2.4 mL) designed for sensitive and high-resolution gel filtration separations of biomolecules in the micropreparative scale. Superdex 200 has a separation range for biomolecules with molecular weights between 10 000 and 600 000.
Application
Columns are not suitable for use with ÄKTAprime plus system.
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
Features and Benefits
- Excellent choice for the polishing step in a purification procedure.
- Precision columns (PC) for micropurification and analysis: sample volumes of 4 - 50 μL.
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- New hardware design for convenient direct connection to high performance chromatography systems such as ÄKTA design.
Preparation Note
Store at 4 to 30 °C (20% ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superdex is a registered trademark of Cytiva
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