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Merck
CN

GE29-0915-97

Superose® 6 Increase 5/150 GL

Cytiva 29-0915-97, 8.6 μm avg. part. size, 5/150 GL

别名:

Superose® 6 Increase

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NACRES:
SB.52
UNSPSC Code:
41115700

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material

glass column, polyethylene filter

product line

Superose®

mol wt

5000-5000000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29-0915-97

parameter

<0.15 mL/min flow rate, <0.75 mL/min flow rate, 3 Mpa (435 psi), 4-50 μL sample volume

bed volume

24 mL

matrix

cross-linked agarose, polymeric

matrix active group

agarose phase

avg. part. size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superose® 6 Increase 5/150 GL is a versatile, prepacked column for rapid purity check and screening by size exclusion chromatography for proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When time is limited and small buffer and sample consumption is important, this is the column of choice.

Application

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Preparation Note

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

存储类别

3 - Flammable liquids

法规信息

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商品

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

实验方案

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

相关内容

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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