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About This Item

UNSPSC代码:
41105323
NACRES:
NA.25
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特点

wetted part: no

制造商/商品名称

Cytiva 80-6483-56

包装

pkg of 500 assays

储存温度

2-8°C

一般描述

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

Common spectrophotometric methods of quantitating protein rely either on Coomassie dye binding or protein-catalyzed reduction of cupric (Cu2+) ion to cuprous (Cu+) ion. Dye-binding assays cannot be used in the presence of any reagent that also binds the dye. This includes carrier ampholytes and detergents such as PlusOne CHAPS, SDS, and Triton X-100. Assays that depend on the reduction of cupric ion cannot be used in the presence of reductants such as DTT, or in the presence of reagents that form complexes with cupric ion, such as thiourea or EDTA.

The 2-D Quant Kit procedure works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein. Precipitated proteins are resuspended in a copper-containing solution and unbound copper is measured with a colorimetric agent. The absorbance at 480 nm is inversely related to the protein concentration. The assay has a linear response to protein in the range of 0 to 50 μg and recommended sample volume is 1 to 50 μ.

特点和优势

  • Accurately determine protein concentration in the presence of 2% SDS, 1% DTT, 8 M urea, 2 M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer.
  • Quantitatively precipitates proteins while leaving interfering substances behind.
  • Linear response in the range of 0 to 50 μg protein, with recommended sample volumes of 1 to 50 μ.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

象形图

Environment

警示用语:

Danger

危险声明

储存分类代码

8B - Non-combustible corrosive hazardous materials


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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