Matrix Metalloproteinase-9 (MMP-9) Human, Biotrak^™ Assay

The Biotrak^™ MMP-9 ELISA provides a simple, specific, and precise quantitative determination of MMP-9 in tissue culture supernatants and homogenates as well as plasma.

The assay is based on a two-site ELISA sandwich format using two antibodies directed against different epitopes of MMP-9. During the first incubation step, MMP-9 present in the samples or the standards is bound to a microplate pre-coated with antibody. During the second incubation step, detection antibody conjugated to horseradish peroxidase is added, which forms an immobilized complex. The amount of peroxidase bound to each well is determined by the addition of tetramethylbenzidine (TMB) ‘ready-to-use′ substrate. The reaction is stopped by the addition of an acid solution and the resultant color measured at 450 nm in a microplate spectrophotometer. The concentration of proMMP-9 in samples is determined by interpolation from a standard curve. MMP-9 may be measured in the range 1–32 ng/mL for tissue culture and the sensitivity of the assay is 0.6 ng/mL.

Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and measurement of 40 unknowns in duplicate. A separate protocol is included for the measurement of MMP-9 in plasma (assay range 4–128 ng/mL). The Biotrak^™ MMP-9 ELISA is suitable for research purposes only.

Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Biotrak MMP-1, MMP-3, MMP-9, and TIMP-1 Human ELISAs
These products are developed in collaboration with FUJI CHEMICAL INDUSTRIES, Ltd.(Japan).

Biotrak is a trademark of Cytiva