usage
kit sufficient for 50 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
UV/Vis spectroscopy: suitable
λmax
340 nm
application(s)
cell analysis
detection
storage temp.
2-8°C
Quality Level
Application
谷氨酰胺和谷氨酸测定试剂盒用于确定以下物质中L-谷氨酰胺的浓度：
- 最低必需培养基（MEM）培养基
- 上清液
- 血浆
该试剂盒通过将NAD还原为NADH，在339 nm波长处测定分光光度，用酶法测定L-谷氨酰胺和L-谷氨酸，
检测范围为：0.056-0.56 mM L-谷氨酸；0.14-1.4 mM L-谷氨酰胺。
检测范围为：0.056-0.56 mM L-谷氨酸；0.14-1.4 mM L-谷氨酰胺。
Biochem/physiol Actions
谷氨酰胺/谷氨酸测定试剂盒设计通过L-谷氨酰胺酶促脱氨、L-谷氨酸脱氢以及NAD+ 转为NADH后，用分光光度法测量L-谷氨酰胺和/或L-谷氨酸。该试剂盒可用于分光光度法测定液体制剂中的L-谷氨酰胺和/或L-谷氨酸。
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
危险化学品
常规特殊物品
此项目有
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Coutts AJ, et al.
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Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。
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