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Merck
CN

GW22423F

Anti-PAFAH1B2 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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NACRES:
NA.41
UNSPSC Code:
12352203
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biological source

chicken

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

manufacturer/tradename

Genway 15-288-22423F

technique(s)

indirect ELISA: suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PAFAH1B2(5049)

General description

PAFAH1B2 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 2) forms a component of platelet activating factor acetylhydrolase (paf-ah), which is composed of a regulatory subunit called Pafah1b1. The other two subunits are catalytic and non-identical and are called Pafah1b2 and Pafah1b3. This protein has a molecular weight of 30kDa. It is alternatively transcribed into two isoforms, which differ at their C-termini. One isoforms is specific to testis.

Immunogen

Immunogen Sequence: GI # 4505585, sequence 1-229
Recombinant platelet-activating factor acetylhydrolase, isoform Ib, β subunit 30kDa

Application

Anti-PAFAH1B2 antibody produced in chicken is suitable for indirect ELISA and western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Platelet-activating factor acetylhydrolase IB subunit β (PAFAH1B2) is a component of plasma PAF-AH, which can hydrolyze oxidized phospholipids and may attenuate the spreading of lipid peroxidation. It plays an important role in various physiological and pathophysiological functions including development, reproduction, inflammation, hemostasis, and apoptosis. It forms a heterodimer with PAFAH1b3, in erythrocytes, and is responsible for rapid hydrolysis of aspirin.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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分析证书(COA)

Lot/Batch Number

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Yutaka Hirashima et al.
Neurosurgery, 70(3), 602-609 (2011-08-26)
Free radicals and lipid peroxidation are thought to be related to the vasospasm generation after subarachnoid hemorrhage (SAH). Plasma platelet-activating factor-acetyl hydrolase (PAF-AH) degrades phospholipids with an oxidatively modified fatty acyl chain. To compare plasma PAF-AH activity and free forms
Gang Zhou et al.
The Journal of biological chemistry, 288(17), 11940-11948 (2013-03-20)
Aspirin is rapidly hydrolyzed within erythrocytes by a heterodimer of PAFAH1b2/PAFAH1b3 but also in plasma by an unidentified activity. Hydrolysis in both compartments was variable, with a 12-fold variation in plasma among 2226 Cleveland Clinic GeneBank patients. Platelet inhibition by
Bruce T Scott et al.
Prostaglandins & other lipid mediators, 85(3-4), 69-80 (2007-12-25)
Platelet activating factor acetylhydrolase (paf-ah), a potent regulator of platelet activating factor activity, plays an important role in various physiological and pathophysiological functions including development, reproduction, inflammation, hemostasis, and apoptosis. Intracellular paf-ah (paf-ah-Ib) is composed of a regulatory subunit, Pafah1b1

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