表单
suspension
质量水平
技术
affinity chromatography: suitable
基质
Highly cross-linked 6% Beaded Agarose
容量
15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
储存温度
2-8°C
一般描述
HIS-Select 高流动性镍亲和凝胶是一种固定化金属离子亲和色谱（IMAC）凝胶，设计用于特异性结合色谱系统中的His-tag蛋白（含组氨酸蛋白质），压力高达 200 psi，最大线性流速为 3,000 cm/hr。
应用
亲和凝胶适用于在天然和变性条件下纯化组氨酸标记的蛋白质（His-tag蛋白）。
镍亲和凝胶用于纯化His-tag® 蛋白（组氨酸标记蛋白） 。 HIS-Select® 高流动性 (HF) 产品将HIS-Select 技术优良的选择性和高度交联的琼脂糖相结合，可在压力下实现更高的流速和机械稳定性。 HIS-Select HF产品设计用于生产规模的纯化和FPLC™ 应用。 和其他HIS-Select产品一样，次氮基三乙酸镍（Ni-NTA)）的螯合基团与琼脂糖的不带电亲水键可确保实现真正的一步纯化。
特点和优势
- 高选择性确保了高纯度
- 独特的不带电亲水键减少了非特异性结合
- 组氨酸标记蛋白（His-tag蛋白）的结合能力大于 15 mg/mL
- 高度交联的琼脂糖基质允许在更高压力下纯化
- 在变性或非变性条件下结合
- 一步纯化
外形
30% 乙醇溶液的混悬液 (1:1)
制备说明
使用前必须除去乙醇。用去离子水洗涤亲和凝胶以除去乙醇，然后用提供的平衡缓冲液进行平衡。
法律信息
FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2
靶器官
Eyes,Central nervous system, Respiratory Tract
WGK
WGK 3
闪点(°F)
88.0 °F
闪点(°C)
31.1 °C
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
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Ivanova, A.S. et al.
Russian journal of developmental biology, 49, 393-397 (2018)
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Nature chemical biology, 12(12), 1089-1096 (2016-10-25)
Cellular signaling is often propagated by multivalent interactions. Multivalency creates avidity, allowing stable biophysical recognition. Multivalency is an attractive strategy for achieving potent binding to protein targets, as the affinity of bivalent ligands is often greater than the sum of
David S Pitcher et al.
EBioMedicine, 2(7), 642-648 (2015-08-20)
The proteasome inhibitor Bortezomib is used to treat multiple myeloma (MM). Bortezomib inhibits protein degradation by inactivating proteasomes' active-sites. MM cells are exquisitely sensitive to Bortezomib - exhibiting a low-nanomolar IC(50) - suggesting that minimal inhibition of degradation suffices to
Irving E Vega et al.
Frontiers in neuroscience, 13, 845-845 (2019-08-29)
The transition of tau proteins from its soluble physiological conformation to the pathological aggregate forms found in Alzheimer's disease and related dementias, is poorly understood. Therefore, understanding the process that modulates the formation of toxic tau oligomers and their conversion
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International journal of biological macromolecules, 106, 1041-1051 (2017-08-31)
l-Asparaginase (EC 3.5.1.1) is an important medical enzyme that catalysis the hydrolysis of l-asparagine to aspartic acid and ammonium. For over four decades l. asparaginase utic agent for the treatment of a variety of lymphoproliferative disorders and lymphoma such as
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利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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