H0656
HCC-1 human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
Hemofiltrate CC chemokine-1, M-CIF, Macrophage Colony Inhibition Factor
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关于此项目
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
8.7 kDa
Impurities:
endotoxin, tested
assay
≥97% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
form
lyophilized powder
potency
1.5-15 EC50 (g/mL)
mol wt
8.7 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CCL14(6358)
Biochem/physiol Actions
HCC-1, a member of the C-C, or β chemokine class, is a 8.7 kDa, non-glycosylated polypeptide of 74 amino acids. Mature HCC-1 is produced by cleavage of a 19 amino acid signal peptide from the precursor HCC-1 (93 amino acids).
HCC-1, a member of the C-C, or β chemokine class, is a 8.7 kDa, non-glycosylated polypeptide of 74 amino acids. Mature HCC-1 is produced by cleavage of a 19 amino acid signal peptide from the precursor HCC-1 (93 amino acids). HCC-1 is expressed constitutively in normal tissues and is present in high concentrations in human plasma. It shows approximately 46% amino acid identity with MIP-1α and MIP-1β, and 29-37% sequence identity with other C-C chemokines.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to chemoattract cultured human monocytes or BaF/3 hCCR1 transfected cells.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
P Schulz-Knappe et al.
European journal of medical research, 1(5), 223-236 (1996-02-22)
The structural determination of circulating human peptides is essential to determine their correct posttranslationally processed form. Human hemofiltrate from patients with end stage renal disease is accessible in large quantities and is used as a source for the preparation of
E C Chew et al.
Anticancer research, 17(5A), 3581-3585 (1997-12-31)
The nuclear matrix is the non-chromatin skeleton of the nucleus. This structure contributes to the shape of the nucleus and regulates various nuclear functions. In this study, nuclear matrix proteins of human normal liver, a liver cancer cell line, HepG2
P Schulz-Knappe et al.
The Journal of experimental medicine, 183(1), 295-299 (1996-01-01)
A novel CC chemokine, HCC-1, was isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a relative molecular mass of 8,673 and consists of 74 amino acids including four cysteines linked to disulfide bonds. HCC-1 cDNA was
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