recombinant
expressed in HEK 293 cells
assay
>95% (SDS-PAGE)
potency
≤0.2 ng/mL EC50
mol wt
33-48 kDa (glycosylated)
impurities
<1 EU/μg endotoxin
storage temp.
−20°C
General description
LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation.
Preparation Note
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1X PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Upon reconstitution, the cytokine can be stored at 4°C for short term only or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.
Analysis Note
The activity was determined by the dose- dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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