biological source
Limulus polyphemus hemolymph
type
Type VIII
assay
≥85% protein basis (biuret)
form
lyophilized powder
composition
Protein, ≥85% biuret
copper content
0.10-0.20% (ICP-atomic emission)
UniProt accession no.
storage temp.
2-8°C
Gene Information
Limulus polyphemus ... LOC(106468801)
General description
鲎（马蹄蟹）血淋巴中的血蓝蛋白是一种被动结合氧的高分子铜蛋白。
来自鲎的血蓝蛋白的结构包括：在 N 末端具有 α-螺旋区的结构域 I，具有活性位点铜残基的结构域 II 和 4 个 α-螺旋束，以及在 C 末端具有反向平行 β-桶结构的结构域 III 。血蓝蛋白的分子量为 72 kDa，具有酚氧化酶功能。
Application
血蓝蛋白用于研究突触成熟过程中 LIM 激酶 1 在突触前末端的积累。 它也被用于使用抗肽抗血清对人卵泡刺激素-β进行地形分析。
鲎血淋巴中的血蓝蛋白已用于：
- 作为电泳场梯度聚焦中优化无色蛋白荧光猝灭的参考标准
- 作为基于 pH 的结晶筛选实验的标准蛋白质
- 肽结合研究中
Biochem/physiol Actions
在马蹄蟹（鲎）中存在着由 48（8×6）个亚基组成的最大血蓝蛋白分子，最多具有八个不同的亚基类型（6× 亚基类型 I、8×II、2×IIA、8×IIIA、8×IIIB、8×IV、4×V、4×VI）。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物来源生物产品
此项目有
Limulus polyphemus hemocyanin: 10 A cryo-EM structure, sequence analysis, molecular modelling and rigid-body fitting reveal the interfaces between the eight hexamers
Martin AG, et al.
Journal of Molecular Biology, 366(4), 1332-1350 (2007)
S J O'Leary et al.
Hearing research, 298, 27-35 (2013-02-12)
This study reviews the cochlear histology from four hearing preservation cochlear implantation experiments conducted on 73 guinea pigs from our institution, and relates histopathological findings to residual hearing. All guinea pigs had normal hearing prior to surgery and underwent cochlear
Will interventional angiology replace vascular surgery?
R L Dalman et al.
Acta chirurgica Scandinavica. Supplementum, 555, 25-35 (1990-01-01)
Bernard R Lauwerys et al.
Arthritis and rheumatism, 65(2), 447-456 (2012-12-04)
We developed interferon-α-kinoid (IFN-K), a drug composed of inactivated IFNα coupled to a carrier protein, keyhole limpet hemocyanin. In human IFNα-transgenic mice, IFN-K induces polyclonal antibodies that neutralize all 13 subtypes of human IFNα. We also previously demonstrated that IFN-K
Victoria A Valdenegro-Vega et al.
Veterinary immunology and immunopathology, 151(1-2), 113-123 (2012-12-05)
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens - dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) - administered using different delivery strategies.
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