一般描述
Heat shock protein 27 (HSP27), also known as mouse HSP25, is a highly conserved oligomeric protein. This protein is expressed constitutively in many cell types and tissues, such as the placenta, breast, skin, platelets, uterus and cervix. HSP27 is expressed in the cytoplasm and localizes to the nucleus upon stress stimuli. The HSPB1 or the HSP27 gene is mapped on the human chromosome at 7q11.23.
特异性
Anti-Heat Shock Protein 27/25 (HSP27/25) recognizes HSP27 (27 kDa) (mouse HSP25).
免疫原
synthetic peptide corresponding to amino acids 190-209 located at the C-terminus of mouse HSP27/25, conjugated to KLH. The sequence is identical in dog and Chinese hamster, highly conserved in rat HSP27 (85% identity), and to a lesser extent in human HSP27 (65%).
应用
Anti-Heat Shock Protein 27/25 antibody produced in rabbit may be used in western blotting and immunofluorescence.
生化/生理作用
Heat shock protein 27 (HSP27) accumulates in cells exposed to a short period of hyperthermia and contributes to the development of a transient state of thermotolerance. This protein is stimulated by various cytokines, growth factors, hormones, and chemicals. HSP27 shows rapid phosphorylation following exposure to stress stimuli. HSP27 acts as an actin-cap binding protein and can inhibit actin polymerization, thus modulating actin dynamics during stress. It also protects against apoptotic cell death triggered by a variety of stimuli including hyperthermia, oxidative stress, Fas ligand, and cytotoxic drugs. HSP27 interferes specifically with the mitochondrial pathway of caspase-induced cell death by acting as a negative regulator of cytochrome c-dependent activation of caspase-3.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilu-tions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
