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安全信息

H4522

Sigma-Aldrich

人血清

from human male AB plasma, USA origin, sterile-filtered

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别名:
人血浆, 人血清
MDL编号:
MFCD00165829
NACRES:
NA.71

生物来源

human male plasma (Type AB)

质量水平

300

无菌性

sterile-filtered

组成

hemoglobin, ≤30 mg/dL

来源

USA origin

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

杂质

≤10 EU/mL endotoxin

运输

dry ice

储存温度

−20°C

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相关类别

应用

人 AB 血清用于组织工程、移植和细胞治疗应用，可用于扩增来自脂肪组织的间充质干细胞 (MSC) 或来自人骨髓的间充质基质细胞； 用于标准化的角膜缘上皮干细胞移植物生成和移植； 用于在造血干细胞扩增培养基中以外周血体外扩增 NK 细胞； 以及移植前人胰岛培养技术的升级。
人血清已被用于：作为含吐温®去污剂的磷酸盐缓冲液 (PBST)的成分用于桥连酶联免疫吸附试验(ELISA); 作为模拟生物样品用于评估复杂生物样品在纸基ELISA试验中的性能; 作为稀释剂用于制备基线样品的稀释系列，从而为添加NISTmAb的血清和多发性骨髓瘤（MM）患者样品MM2232生成标准曲线

生化/生理作用

人AB血清通常是通过全血自然凝结而获取，称为Off-The-Clot (OTC) 血清。由凝结全血获取的人血清是首选的血清材料，因为它通常含有较高浓度的更加多样化的生长因子，可实现最优的细胞生长。来自AB 血型供体的人血清没有A型和B型血抗原的特异性抗体。这一特性令其成为通用的供体血清，适用于各种细胞培养，包括人干细胞系。

包装

由于产品包装瓶供应不足，不同批次产品的包装可能不同。瓶盖是否包裹热缩膜不会影响产品的完整性/无菌性。

警告

ELISA 检测每位捐献者的乙型&和丙型肝炎无反应，对人类免疫缺陷病毒 (HIV) 抗体无反应。然而，应将人源产品视为潜在传染性产品并进行相应处理。

分析说明

内毒素检测

其他说明

我们的人血清产品呈浑浊状并不罕见。该产品来自混合血清，浑浊表明血清中存在脂质。暂无透明、无脂质的人血清产品。

法律信息

TWEEN is a registered trademark of Croda International PLC

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--人源产品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

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部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. There is a precipitate in my serum.  Is my serum contaminated?

    Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. Is Product H4522, Human serum, Heat Inactivated (HI)?

    This serum is not heat inactivated. If heat inactivated serum is needed, the serum can be heated to 56 °C for 30 minutes. Heating inactivates the complement cascade. Active complement can participate in cytolytic events, leading to contraction of smooth muscle, histamine release from mast cells and platelets, and activation of lymphocytes and macrophages.

  5. How is Product H4522, Human serum, prepared?

    Blood is collected and has citrate added as an anticoagulant to inhibit clotting.  The blood is then centrifuged and separated into cells and plasma.  The plasma is separated from the cells, and calcium is added to the plasma to activate the clotting cascade.  The preparation is then centrifuged again, and the serum is the liquid product remaining.  This process yields a serum low in growth factors, as it does not contain those factors released from the cells upon clotting.

  6. Product H4522, Human serum, is cloudy. What causes the cloudiness?

    It is not unusual for our human serum products to be cloudy. This product is from pooled serum and cloudiness indicates that lipids are present in the serum. No clear, lipid-free human serum products are available.

  7. Why do we specify male AB in the description of Product H4522, Human serum?

    Male blood is used in serum products, where specified, because female serum can have more antibodies resulting from pregnancy.  Type AB serum is used as there are no anti-A and/or anti-B antibodies that are found in other blood types.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?

    Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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