biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
15-60 ng/mL ED50
mol wt
dimer 30-45 kDa (glycosylated)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
−20°C
Biochem/physiol Actions
FGF-8b belongs to the FGF protein family. FGF-8b is important in embryogenesis, in pathways that lead to left-right determination and coordinate the vertebral segmentation process, and in processes that activate Homeobox genes. There are several isoforms of FGF-8 produced by alternative splicing.
Preparation Note
HumanKine Fibroblast Growth Factor-8b, expressed in human HEK 293 cells is a glycosylated monomer with an apparent molecular mass of 30-45 kDa due to glycosylation. Production in human HEK 293 cells offers authentic glycosylation, which is absent when this cytokine is expressed in E. coli. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of the BALB/3T3 cell line.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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商品
Fibroblast growth factors in cell culture and various growth factors for your research
用于细胞培养的成纤维细胞生长因子，以及用于您的研究的各类生长因子
相关内容
Instruction
Maija P Valta et al.
Journal of cellular biochemistry, 107(4), 769-784 (2009-05-06)
Fibroblast growth factor 8 (FGF-8) is expressed at an increased level in a high proportion of prostate cancers and it is associated with a poor prognosis of the disease. Our aim was to study the effects of FGF-8b on proliferation