H6537
Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse
~1 mg/mL, clone HDAC3-83, purified immunoglobulin, buffered aqueous solution
别名:
Anti-HD3
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关于此项目
Conjugate:
unconjugated
Clone:
HDAC3-83, monoclonal
Application:
ARR, WB
Citations:
1
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HDAC3-83, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
mouse, human
concentration
~1 mg/mL
technique(s)
microarray: suitable, western blot: 1 μg/mL using total cell extracts of NIH3T3 cells
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HDAC3(8841)
mouse ... Hdac3(15183)
General description
Monoclonal Anti-Histone Deacetylase 3 (HDAC3) (mouse IgM isotype) is derived from the HDAC3-83 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. The histone deacetylase 3 (HDAC3) gene is located on the human chromosome at 5q31.3. HDAC3 protein belongs to the class I HDAC.
Immunogen
synthetic peptide corresponding to amino acids 411-428 of human and mouse HDAC3.
Application
Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
Histone deacetylase 3 (HDAC3) protein is required for DNA replication, maintaining chromatin structure, genomic stability, and DNA damage control. This protein plays a role in eliminating histone acetylation. HDAC3 protein co-works with nuclear hormone corepressors, such as nuclear receptor corepressor (NCOR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT). HDAC3 protein levels are high in proliferating cells of hepatocellular carcinoma.
Monoclonal Anti-Histone Deacetylase 3 (HDAC3) recognizes human and mouse HDAC3 (approx. 50 kDa).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Srividya Bhaskara et al.
Cancer cell, 18(5), 436-447 (2010-11-16)
Hdac3 is essential for efficient DNA replication and DNA damage control. Deletion of Hdac3 impaired DNA repair and greatly reduced chromatin compaction and heterochromatin content. These defects corresponded to increases in histone H3K9,K14ac; H4K5ac; and H4K12ac in late S phase
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