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HLP602

Normal Human Sinusoidal Endothelial Cells

Name: Normal Human Sinusoidal Endothelial Cells
Brand: SIGMA

Single-Donor Cryopreserved Sinusoidal Endothelial cells isolated from human livers, high viability, and characterized by cell specific markers

别名:

Homogenous population isolated from whole human livers

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关于此项目

NACRES:

NA.81

UNSPSC Code:

41106509

Biological source:

human liver

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属性

biological source

human liver

form

frozen liquid

packaging

pkg of 1 vial (1 vial contains ≥1 million cells), pkg of 1 vial contains ≥0.5 million cells

technique(s)

cell culture | mammalian: suitable

color

cloudy

storage temp.

-140 to -196°C

说明

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Human hepatic Sinusoidal Endothelial cells (HSECs) are specialized endothelial cells in the liver that participate in the receptor-mediated clearance of endotoxin, bacteria, and other compounds. HSECs are also known to regulate inflammation, leukocyte recruitment, and host-immune responses to invading pathogens. Human HSECs are a valuable tool for the study of liver physiology and pathophysiology in vitro.

Application

Human hepatic sinusoidal endothelial cells are ideal in studies of: chronic liver injury, toxicity, immune response, portal vein fibrosis, angiogenesis, liver regeneration, stellate cell activation and more. We offer matched sets of sinusoidal endothelial cells from same donor as Hepatocytes, which can be used for co-culture systems to better simulate the complex microenvironment of the liver and gain insights into various liver-related diseases and conditions.

Features and Benefits

Our collection of hepatic Sinusoidal Endothelial cells is isolated through collagenase digestion and selective cell culture media. Our frozen HSECs are cryopreserved at the end of the primary culture. Each lot undergoes testing for specific cell markers and comes with a guarantee of ≥70% post-thaw viability. In addition, all cells have been tested for the absence of HIV, HBV, HCV, Syphillis and microbial contaminants (fungi, bacteria, and mycoplasma).

Disclaimer

Each donor is tested and found non-reactive to viral DNA from HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C. However, no known test can offer complete assurance that the viruses that cause HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C are not present. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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实验方案

Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

Primary Human Hepatocytes Culture Protocol

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Primary Human Hepatic Kupffer Cell Culture Protocol

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

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Frequently Asked Questions (FAQs) about Hepatocytes and Other Liver Cells

Explore answers to frequently asked questions (FAQs) about culturing primary human hepatocytes and other liver cells including Stellate cells, Kupffer cells, Sinusoidal Endothelial cells, and more.