产品线
MISSION®
表单
liquid
浓度
≥1x106 VP/ml (via p24 assay)
技术
capture ELISA: 106 TU/mL using p24 (Volume 200 uL)
成熟序列
UACUGCAUCAGGAACUGAUUGGA
Sanger mature/minor 登记号
Sanger microRNA登记号
运输
dry ice
储存温度
−70°C
一般描述
Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.
- Allows for potent inhibition of the desired miRNA
- Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
- Enables long-term inhibition without repeat transfection
其他说明
Based on miRBase V19 Mature ID
法律信息
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Ai-Na Liu et al.
Journal of cellular and molecular medicine, 22(9), 4034-4044 (2018-06-24)
We tried to identify the function of LINC01614 in lung adenocarcinoma (LUAD) and reveal its underlying mechanisms. qRT-PCR was applied to assess the expression of LINC016014 in LUAD tissues, noncancerous tissues and cells. Through colony formation assay, MTT assay and
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持