Anti-ATAD2 antibody produced in rabbit

ATAD2 (ATPase family, AAA domain containing 2) is an evolutionarily conserved AAA+ nuclear protein consisting of a bromodomain and a double AAA⁺ class ATPase domain. It is localized in in the nucleus with a molecular mass of ~170kDa. Its expression has been observed in different types of cancerous cells such as human breast cancer. It is mapped to chromosome 8q24.13.

ATPase family AAA domain-containing protein 2 recombinant protein epitope signature tag (PrEST)

Anti-ATAD2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

ATAD2 (ATPase family, AAA domain containing 2) is associated with various cellular processes such as DNA replication, chromatin remodeling, transcription, and DNA damage. In the E2-Induced cell Proliferation and cell cycle progression, ATAD2 functions as an ERα (estrogen receptor) coactivator and facilitates E2-stimulated expression of cell cycle regulators by protein complex-remodeling phenomena. It also plays a vital role in the tumorigenesis. ATAD2 alters chromatin accessibility at MYC target loci by directly interacting with MYC as a cofactor. The chromatin accessibility further modifies transcriptional expression of the MYC gene.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Corresponding Antigen APREST74648

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.