biological source
mouse
recombinant
expressed in NSO cells
assay
≥90% (SDS-PAGE)
form
lyophilized powder
potency
0.5-2.5 μg/mL ED50
mol wt
calculated mol wt 28.9 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Igfbp5(16011)
General description
IGFBP5 (insulin-like growth factor binding protein-5) is a secreted protein and can also move to the nucleus. IGFBPs have cysteine-rich amino- and carboxyl-terminal domains.
Application
As a result of glycosylation, the recombinant mouse protein migrates to at least three bands with molecular weights ranging from 28-38 kDa in SDS-PAGE under reducing conditions.
Biochem/physiol Actions
Binds to bone cells and is the predominant insulin-like growth factor binding protein in bone; when binding the extracellular matrix, it modulates IGF activity.
IGFBP-5 binds to bone cells and is the predominant insulin-like growth factor binding protein in bone. When IGFBP-5 binds the extracellular matrix, it modulates IGF activity. Mouse, human, and rat IGFBP-5 share 97% homology.
IGFBPs (insulin-like growth factor binding proteins) control IGF functions by regulating their access to other proteins, including receptors. IGFBP5 is involved in osteogenesis, muscle differentiation and kidney development. It is a crucial protein for the development of fibrosis.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 1.25 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to inhibit recombinant mouse IGF-II on MCF-7 cells.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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相关内容
Datasheet
Hidekata Yasuoka et al.
PloS one, 9(2), e87754-e87754 (2014-02-20)
Systemic sclerosis (SSc) is characterized by excessive fibrosis of the skin and internal organs due to fibroblast proliferation and excessive production of extracellular matrix (ECM). We have shown that insulin-like growth factor binding protein (IGFBP)-5 plays an important role in
Mahta Nili et al.
The Journal of biological chemistry, 287(2), 1510-1519 (2011-11-26)
The six high-affinity insulin-like growth factor-binding proteins (IGFBPs) comprise a conserved family of secreted molecules that modulate IGF actions by regulating their half-life and access to signaling receptors, and also exert biological effects that are independent of IGF binding. IGFBPs
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| I3782-25UG | 04061833856369 |