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Merck
CN

I5284

异淀粉酶 来源于假单胞菌

ammonium sulfate suspension, ≥3,000,000 units/mg protein

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UNSPSC Code:
12352204
MDL number:
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form

ammonium sulfate suspension

specific activity

≥3,000,000 units/mg protein

storage temp.

2-8°C

Biochem/physiol Actions

催化 (1-6)- α 的水解糖原、支链淀粉及其 β 中的 -D-糖苷键-极限糊精。

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical form

Supplied as a suspension in 3.2 M ammonium sulfate

Other Notes

One unit causes an increase in A610 of 0.1 in 1 hour using rice starch as a substrate.

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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分析证书(COA)

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Steven S-S Wang et al.
Bioprocess and biosystems engineering, 33(8), 1007-1015 (2010-04-08)
The current research examines the impact of agitation on deactivation of isoamylase and β-amylase under supercritical carbon dioxide (SC-CO2). Our experimental results showed that the activity of either enzyme decreased with increasing pressure or speed of agitation. The degree of
Francisco J Cinco-Moroyoqui et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 150(2), 153-160 (2008-04-09)
Three isoamylases of Rhyzopertha dominica (termed RdA70, RdA79, and RdA90 according to their relative mobility in gel electrophoresis) were isolated by ammonium sulfate fractionation and hydrophobic interaction chromatography. RdA70 and RdA79 showed an optimal pH of 7.0, whereas for RdA90
Rupendra Mukerjea et al.
Carbohydrate research, 345(3), 449-451 (2009-12-26)
Amylose, a putative linear alpha-(1-->4)-glucan and a component of most starches, was isolated from potato, rice, and wheat starches by forming the 1-butanol complex in a solution of the starches. It previously had been found that these amyloses were incompletely
Sebastian Streb et al.
The Journal of biological chemistry, 287(50), 41745-41756 (2012-09-29)
In this study, we investigated which enzymes are involved in debranching amylopectin during transient starch degradation. Previous studies identified two debranching enzymes, isoamylase 3 (ISA3) and limit dextrinase (LDA), involved in this process. However, plants lacking both enzymes still degrade
Francisco Vilaplana et al.
Journal of chromatography. A, 1218(28), 4434-4444 (2011-06-07)
Instrumental and procedural optimizations are developed for a new method to obtain 2-dimensional distributions for branched homopolymers based on size and branching. The method uses 2-dimensional off-line size-exclusion chromatography (SEC) and chemical debranching, in this case using debranching enzymes on

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