form
aqueous glycerol suspension
extent of labeling
1-2 mg per mL
matrix
4% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
α-amino
matrix spacer
16 atoms
storage temp.
−20°C
Physical form
Suspension in 50% glycerol, 0.15 M NaCl, 0.01 M Tris, pH 7.4, 0.4 mM ZnCl2
Preparation Note
Insulin (I 3505) coupled preferentially through the alpha amino via active ester to epichlorohydrin-activated di(aminopropylamino)succinyl
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Y Fujita-Yamaguchi et al.
The Journal of biological chemistry, 258(8), 5045-5049 (1983-04-25)
Insulin receptor was purified 2400-fold with an overall yield of 40% from human placental membranes by affinity chromatography on wheat germ agglutinin-Sepharose and insulin-Sepharose. The receptor was eluted from insulin-Sepharose using mild conditions, eliminating urea, so that it was stable
Affinity chromatography of insulin receptors.
P Cuatrecasas et al.
Methods in enzymology, 34, 653-670 (1974-01-01)