Phagocytosis Assay Kit

Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their e+D10ngulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae) and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.

The Phagocytosis Assay Kit (Red Zymosan) uses fluorescently pre-labeled Zymosan particles to detect and quantify in vitro phagocytosis by fluorescent microscope, spectrophotometer (Ex/Em = 540/570 nm) or flow cytometry.

This kit is suitable for screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells.