column volume
2.5 mL
storage temp.
2-8°C
Application
Iminodiacetic acid-agarose is used in affinity chromatography, protein chromatography and chelating resins.
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Datasheet
Instructions
S Ghosh et al.
Gene, 176(1-2), 249-255 (1996-10-17)
Vectors have been constructed for the general purpose of expressing foreign proteins in E. coli. These vectors allow the production in high yield of either native proteins or of fusion proteins which contain, at their amino terminus, the peptide Met
S S Lam et al.
Biochemical and biophysical research communications, 253(1), 135-142 (1999-01-06)
Two ribosome inactivating proteins designated alpha- and beta-pisavins were isolated from seeds of the garden pea Pisum sativum var. arvense Poir with a procedure involving affinity chromatography on Affi-gel Blue gel, immobilized metal ion affinity chromatography on Iminodiacetic acid-agarose, cation
Eva Zatloukalová et al.
Journal of separation science, 29(8), 1082-1087 (2006-07-13)
A method for the immobilization of antibodies to inert matrix represents an important factor that affects results of immunoaffinity chromatography. Binding antibodies to immobilized metal ions is an example of oriented immobilization that avoids a random coupling of a protein.