等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
77,000 U/mg
浓度
2,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
应用
Suitable for:
- Removing uracil from DNA
- Control of carry-over contamination in PCR
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
外形
Supplied in 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.5 @ 25° C
其他说明
1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37° C in 1X UDG Reaction Buffer.
Source of protein: A recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12.
Supplied with:KEM0001B (10X UDG Reaction Buffer)
Unit Size: 10,000 U
储存分类代码
10 - Combustible liquids
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