grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
100,000 U/mg
concentration
100,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
Exonuclease III is a 3′ → 5′ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III breaks phosphodiester bonds on the 5′ side of AP sites in both dsDNA and ssDNA, removes 3′ terminal groups on dsDNA, increases MutY turnover, and efficiently degrades 3′ recessed but not 3′ protruding DNA ends (creating 5′ overhangs). Exo III removes a limited number of nucleotides per binding event, resulting in coordinated progressive deletions within the population of DNA molecules.
Application
Suitable for removal of nucleotide(s) from duplex DNA in 3′ – 5′ direction.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 25 mM Tris-HCl, 50 mM KCl, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 8.0 @ 25° C.
Other Notes
1 unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in 30 minutes at 37° C.
Source of protein: Purified from a strain of E. coli that expresses the recombinant Exonuclease III gene.
Supplied with:KEM0004B (10X Yellow Buffer)
Unit size: 50,000 U
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持