等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
80,000 U/mg
浓度
5,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
Lambda Exonuclease is a highly processive 5′ → 3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will no initiate at a nick or gap.
应用
Suitable for applications requiring a highly processive 5′ – 3′ exonuclease which is active on both blunt and 5′ recessed ends.
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
外形
Supplied in 25 mM Tris-HCl, 50 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol at pH7.5 @ 25° C.
其他说明
1 unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37° C.
Source of protein: Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.
Supplied with:KEM0005B (10X Lambda Exo Reaction Buffer)
Unit size: 10,000 U
储存分类代码
10 - Combustible liquids
法规信息
新产品
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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