等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
浓度
10,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and-single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities.
应用
Suitable for:
- End-labeling DNA or RND for probes and DNA sequencing
- Addition of 5′ phosphates to oligonucleotides to allow subsequent ligation
- Removal of 3′ phosphoryl groups
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
外形
Supplied in 10 mM Tris-HCl, 50 mM KCl, 0.1 μM ATP, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.6 @ 25° C.
其他说明
1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37° C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Supplied with:KEM0006B (10X T4 Polynucleotide Kinase Buffer)
Unit size: 10,000 U
储存分类代码
10 - Combustible liquids
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